Oestrogen receptor assay of cryostat sections of human breast carcinomas with simultaneous quantitative histology.
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چکیده
منابع مشابه
Oestrogen receptor assay of cryostat sections of human breast carcinomas with simultaneous quantitative histology.
Cryostat sections of unfixed human breast carcinomas were assayed for oestrogen receptor (ER) content using an isoelectric focusing method to separate the receptor-bound oestradiol. Adjacent sections from the same tissue block were stained so that the tumour content could be estimated by point counting and the ER concentration adjusted to compensate for variations in cellularity. Elastosis was ...
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' Martuza RL, Miller DC, MacLoughlin DT. Estrogen and progestin binding by cytosolic and nuclear fractions of human meningiomas. J Neurosurg 1985;62:750-6. 2Van Netten JP, Algard FT, Coy P, et al. Estrogen receptor assay on breast cancer microsamples. Implications of percent carcinoma estimation. Cancer 1982;49:2383-8. 3Ironside JW, Battersby RDE, Dangerfield VJM, Parsons MA, Timperley WR, Unde...
متن کاملQuantification of oestrogen receptors in breast cancer: radiochemical assay on cytosols and cryostat sections compared with semiquantitative immunocytochemical analysis.
A radiochemical oestrogen receptor assay on cytosol was correlated with a radiochemical and an immunohistochemical oestrogen receptor assay using cryostat sections from 50 breast cancer specimens. Oestrogen receptors were reliably quantitated in 6 micron cryostat sections with Scatchard analysis using radiolabelled oestradiol, and a good quantitative and qualitative relation with cytosol oestro...
متن کاملComparison of new immunohistochemical assay for oestrogen receptor in paraffin wax embedded breast carcinoma tissue with quantitative enzyme immunoassay.
AIM To validate the use of a new mouse monoclonal antibody (1D5) directed against the N-terminal domain (A/B region) of the oestrogen receptor in an immunohistochemical assay (ER-IHA) for paraffin wax embedded tissue. METHODS Breast cancer specimens were surgically obtained from 119 previously untreated patients. For comparison, oestrogen receptor was measured from cytosol fractions using an ...
متن کاملEnzyme histochemical analysis on cryostat sections of human bone marrow.
A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether enzyme-histochemical techniques are also feasible on such material. A selected group of enzymes, some of which are inhibited or destroyed in paraffin- or plastic-embedded samples, have been demonstrated. The...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1983
ISSN: 0021-9746
DOI: 10.1136/jcp.36.4.399